| Ames Trail System | Within Ames | 55 | Asphalt/Concrete |
| Ankeny Trail System | Within Ankeny | 85 | Asphalt/Concrete |
| Cedar River Trail | Within Cedar Rapids | 14 | Asphalt/Concrete |
| Cedar Valley Trails | Within Cedar Falls and Waterloo | 125 | Asphalt/Concrete |
| Cedar Valley Nature Trail | Evansdale to Hiawatha | 54 | Asphalt |
| Chichaqua Valley Trail | Bondurant to Baxter | 28 | Asphalt |
| Cinder Path Trail | Chariton to Humeston | 16 | Cinder/Limestone |
| Clive Greenbelt Trail | Within Clive | 9 | Asphalt |
| Comet Trail and Wolf Creek Trail | Conrad to Gladbrook | 9 | Limestone/Asphalt |
| Council Bluffs Trail System | Within Council Bluffs | 45 | Asphalt |
| Discovery Trail/Clinton/Camanche Trail | Eagle Point Park in Clinton to west end of Camanche | 14 | Asphalt/Concrete |
| Duck Creek Parkway | Davenport to Bettendorf | 14 | Asphalt/Concrete |
| Flint River Trail | Des Moines County/ Burlington | 12 | Concrete/Limestone |
| Gay Lea Wilson Trail | Segments in Altoona, Pleasant Hill, Des Moines, and Ankeny | 35 | Asphalt |
| Great Western Trail | Des Moines to Martensdale | 18 | Asphalt |
| Heart of Iowa Nature Trail | Slater to west of Collins/east of Collins to Rhodes | 27 | Limestone |
| Heritage Trail | Dubuque to Dyersville | 29 | Limestone |
| High Trestle Trail | Ankeny to Woodward | 25 | Asphalt |
| Hoover Nature Trail | Segments from Cedar Rapids to Burlington | 24 | Limestone |
| Iowa Great Lakes Recreational Trail | Within Spirit Lake, Okoboji, Arnolds Park, Milford, Wahpeton, and West Okoboji | 50 | Asphalt |
| Iowa River Corridor Trail | Within Iowa City | 18 | Asphalt/Concrete |
| Jefferson County Trail System | Throughout Jefferson County, near Fairfield | 18 | Limestone/Concrete |
| Jordan Creek Greenbelt Trail | Within West Des Moines | 12 | Asphalt/Concrete |
| Kewash Nature Trail | Keota to Washington | 14 | Limestone |
| Lamoni Recreational Trail | Within Lamoni | 6 | Concrete |
| Le Mars Recreational Trail | Within Le Mars | 7 | Asphalt |
| Linn Creek Greenbelt Parkway | Within Marshalltown | 12 | Asphalt |
| Mahaska Community Recreational Trail | Within Oskaloosa (some on road) | 12 | Concrete |
| Mississippi Riverfront Trail | Within Davenport and Bettendorf | 18 | Asphalt/Concrete |
| Neal Smith Trail/Pat Dorrian Trails | Saylorville/Big Creek area to Hawthorne Park in Des Moines | 28 | Asphalt |
| North Ridge Park Trail | Within Coralville/North Liberty | 10 | Asphalt/Concrete |
| Old Creamery Trail | Vinton to Dysart | 15 | Limestone |
| Ottumwa Trail System | Along the Des Moines River in Ottumwa | 16 | Asphalt/Limestone |
| Park to Park Trail | McKinley Park in Creston to south end of Green Valley Lake State Park | 13 | Asphalt |
| Pioneer Trail | Holland to Reinbeck | 12 | Limestone |
| Prairie Farmer Recreational Trail | Calmar to Cresco | 20 | Asphalt |
| Raccoon River Valley Trail | Jefferson to Clive (Waukee to Herndon Loop) | 90 | Asphalt |
| River City Greenbelt/Winnebago Trails | Within Mason City | 13 | Concrete |
| Rolling Prairie Trail | Allison to Shell Rock; Waverly to Readlyn | 34 | Asphalt |
| Sauk Rail Trail | Lake View to Carroll | 35 | Asphalt |
| Sioux City Trail System | Within Sioux City | 26 | Asphalt/Concrete |
| Solon Trail | Solon to Lake Macbride | 5 | Gravel |
| Summerset Trail | Indianola to Carlisle | 14 | Asphalt |
| T-Bone Trail | Audubon to Atlantic | 21 | Asphalt |
| Three Rivers Trail | Rolfe to west of Eagle Grove | 35 | Limestone |
| Trolley Trail/Clear Lake Trail | Ventura to Mason City | 23 | Asphalt |
| Trout Run Trail | Within Decorah | 13 | Asphalt |
| Twin Lakes Recreation Trail | Around Twin Lakes in Calhoun County (some on road) | 11 | Concrete |
| Volksweg Trail | Pella to Red Rock Reservoir | 14 | Asphalt |
| Wabash Trace Nature Trail | Council Bluffs to Blanchard | 64 | Limestone/Asphalt |
| Wapsi-Great Western Line Trail | Howard to Mitchell counties near Riceville | 17 | Asphalt |
